96-well Genomic DNA Extraction Kit

Aplikace
PCR AFLP, RFLP Southern blotting, real-time PCR

Formát
96-jamkové destičky

96-Well Genomic DNA Extraction Kit je speciálně navržený pro čištění celkové DNA (včetně genomové, mitochondriální a virové DNA) z různých zvířecích vzorků, včetně krve, tělních tekutin, tkáních nebo kultivovaných buněk. Způsob použití proteinázy K a chaotropních solí. Celý proces trvá méně než 60 minut. Více naleznete na stránkách výrobce Favorgen.

FavorPrep™ 96-Well Genomic DNA Kit is specially designed for purification of total DNA (including genomic, mitochondrial, and viral DNA) from a wide variety of animal samples. Each sample type uses specialized lysis procedure and optimized lysis buffer to enhance proteinase K activity. DNA in chaotropic salt is bound to special 96-Well Filter plate in a batch. After washing and elution procedure, the purified DNA is ready-to-use for multiple applications, such as qPCR and library preparation in NGS sequencing.

«Time Saving: Rapid isolation of genomic DNA from tissue samples within 90 mins (depending on the sample type).

«Quick: The procedure allows parallel purification up to 96 samples.

«Flexibility: Extraction of genomic DNAs from various types of tissue, cultured cells or whole blood, buffy coat, body fluids, etc.

«Safe: No caustic organic compounds
Format/Principle: 96- well DNA binding plate (silica membrane)

Sample Size: Up to 200 µl fresh/frozen whole blood, buffy coat, serum, plasma, body fluids; up to 25 mg animal tissue; up to 5×106 animal cultured cells.

Plate Applicability: Vacuum and centrifugation

Operation Time: Within 90 mins/96 preparations

Binding Capacity: Up to 30 µg/well

Elution Volume: 75~200 µl

Expected Yield: 4~12 µg whole blood; 15~20 µg animal culture cells; 5~30 µg animal tissue.